gfp trap magnetic agarose beads (Proteintech)
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gfp trap magnetic agarose beads
Gfp Trap Magnetic Agarose Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+trap+magnetic+agarose+beads/TNFSF14+Antibody/pm40885732-335-16-20
Average 97 stars, based on 264 article reviews
Gfp Trap Magnetic Agarose Beads, supplied by Proteintech, used in various techniques. Bioz Stars score: 97/100, based on 264 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+trap+magnetic+agarose+beads/TNFSF14+Antibody/pm40885732-335-16-20
Average 97 stars, based on 264 article reviews
gfp trap magnetic agarose beads - by Bioz Stars,
2026-11
97/100 stars
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Incubation:Article Title: Phytochrome B and phytochrome-interacting-factor4 modulate tree seasonal growth in cold environments. Article Snippet: Total protein was isolated with extraction buffer (EB) containing 25mM Tris-HCl pH 7.8, 10mM MgCl2, 150mM NaCl, 200mM PMSF, 1μg/ml aprotinin, 0.2% NP40, 40μM MG132, and protease inhibitors (Protease Inhibitor Cocktail for plant cells, Sigma). .. For co-immunoprecipitation assays, 500μg of total proteins were incubated for 3 h with 20 μl of Article Title: Phytochrome B and phytochrome-interacting-factor4 modulate tree seasonal growth in cold environments Article Snippet: Total protein was isolated with extraction buffer (EB) containing 25 mM Tris-HCl pH 7.8, 10 mM MgCl 2 , 150 mM NaCl, 200 mM PMSF, 1 μg/ml aprotinin, 0.2% NP40, 40 μM MG132, and protease inhibitors (Protease Inhibitor Cocktail for plant cells, Sigma). .. For co-immunoprecipitation assays, 500 μg of total proteins were incubated for 3 h with 20 μl of Article Title: An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis Article Snippet: In order to pull down GFP-FMNL1 for mass spectrometry, transiently transfected HeLa cells were were scraped into lysis buffer (50 μl/ml NP-40, 5 mM HEPES, 10 mM NaCl, 0.2 mM DTT, 0.001% Brij 35, 0.01 mM EGTA, 0.01 mM MnCl 2 , 5% glycerol, pH 7.5, 1X Halt protease and phosphatase inhibitor cocktail from Thermo Scientific #78440). .. Then sample was centrifuged at 10,000 X g for 10 min. After washing Article Title: An AGO10:miR165/6 module regulates meristem activity and xylem development in the Arabidopsis root. Article Snippet: For GFP:AGO1, GFP:AGO10, and GFP:P19 IPs, lysates were first pre-cleared with 40 μL of agarose beads (Merck Roche) for 1 h on a rotating wheel at 4 °C. .. Pre-cleared lysates were then incubated for 1 h on a rotating wheel with 30 μL of Article Title: An AGO10:miR165/6 module regulates meristem activity and xylem development in the Arabidopsis root Article Snippet: For GFP:AGO1, GFP:AGO10, and GFP:P19 IPs, lysates were first pre-cleared with 40 μL of agarose beads (Merck Roche) for 1 h on a rotating wheel at 4 °C. .. Pre-cleared lysates were then incubated for 1 h on a rotating wheel with 30 μL of Lysis:Article Title: An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis Article Snippet: In order to pull down GFP-FMNL1 for mass spectrometry, transiently transfected HeLa cells were were scraped into lysis buffer (50 μl/ml NP-40, 5 mM HEPES, 10 mM NaCl, 0.2 mM DTT, 0.001% Brij 35, 0.01 mM EGTA, 0.01 mM MnCl 2 , 5% glycerol, pH 7.5, 1X Halt protease and phosphatase inhibitor cocktail from Thermo Scientific #78440). .. Then sample was centrifuged at 10,000 X g for 10 min. After washing |